Rapid, Flow Cytometric Assay for NK Alloreactivity Reveals Exceptions to Rules Governing Alloreactivity
نویسندگان
چکیده
منابع مشابه
Predicting Alloreactivity in Transplantation
Human leukocyte Antigen (HLA) mismatching leads to severe complications after solid-organ transplantation and hematopoietic stem-cell transplantation. The alloreactive responses underlying the posttransplantation complications include both direct recognition of allogeneic HLA by HLA-specific alloantibodies and T cells and indirect T-cell recognition. However, the immunogenicity of HLA mismatche...
متن کاملAlloreactivity-Based Medical Conditions
1 Sheikh Zayed Institute for Pediatric Surgical Innovation, Children’s National Medical Center, Washington, DC 20010, USA 2Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, MO 66160, USA 3Department of Pediatrics, University of Washington, Seattle Children’s Hospital, Seattle, WA 98109, USA 4Department of Physiology and Immunology, University of Rijeka, 5...
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The contribution of natural CD4(+)CD25(+) regulatory T cells (nTregs) in controlling graft rejection and the mechanism used remain controversial. Using the duality of the 2.102 TCR Ag recognition, we were able to study, for the first time to our knowledge, the involvement of nTregs in the two pathways of allorecognition in a murine adoptive transfer model in which TCR-transgenic nTregs were or ...
متن کاملMeasurement of T Cell Alloreactivity Using Imaging Flow Cytometry
The measurement of immunological reactivity to donor antigens in transplant recipients is likely to be crucial for the successful reduction or withdrawal of immunosuppression. The mixed leukocyte reaction (MLR), limiting dilution assays, and trans-vivo delayed-type hypersensitivity (DTH) assay have all been applied to this question, but these methods have limited predictive ability and/or signi...
متن کاملMolecular and genetic basis for strain-dependent NK1.1 alloreactivity of mouse NK cells.
NK1.1 alloantigen expression can be used to define NK cells in certain mouse strains, such as B6 (NKR-P1C) and SJL (NKR-P1B). However, BALB/c NK cells do not react with the anti-NK1.1 mAb, PK136. To investigate the NK1.1(-) phenotype of BALB/c NK cells, we have undertaken NK1.1 epitope mapping and genomic analysis of the BALB/c Nkrp1 region. Bacterial artificial chromosome library analysis reve...
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ژورنال
عنوان ژورنال: Biology of Blood and Marrow Transplantation
سال: 2010
ISSN: 1083-8791
DOI: 10.1016/j.bbmt.2009.10.026